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Insight into topological and functional relationships of cytochrome c oxidase subunit I of Saccharomyces cerevisiae by means of intragenic complementation
Author(s) -
Meunier Brigitte,
Coster Françoise,
Lemarre Philippe,
Colson Anne-Marie
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80099-g
Subject(s) - complementation , cytochrome c oxidase , protein subunit , mutant , saccharomyces cerevisiae , genetics , yeast , gene , biology , amino acid , oxidase test , biochemistry , enzyme
In yeast, revertants were selected from four respiratory deficient mutants carrying mutations in the cytochrome c oxidase subunit I gene. Intragenic second site mutations revealed amino acids which are functionally complementary to the original mutated position and may be in topological interaction with it. The results provide additional data in favour of the model proposed for the structure of the binuclear centre in proton‐motive oxidases.