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Cloning and sequencing of rat liver cDNAs encoding the regulatory protein of glucokinase
Author(s) -
Detheux Michel,
Vandekerckhove Joël,
Van Schaftingen Emile
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80089-d
Subject(s) - microbiology and biotechnology , recombinant dna , biology , western blot , glucokinase , northern blot , cloning (programming) , molecular cloning , amino acid , gene , escherichia coli , rna , biochemistry , complementary dna , computer science , programming language
cDNAs encoding the rat liver regulatory protein of glucokinase were cloned and sequenced. The deduced protein contains 568 amino acids for a molecular mass of 62,867 Da. Northern blot analysis showed the presence of a major RNA species of 2.35 kb in rat liver. No signal was observed with muscle, brain, heart, testis, intestine or spleen RNA. Recombinant regulatory protein expressed in Escherichia coli was insoluble and inactive, and was presumably contained in inclusion bodies. Western blot analysis showed that the recombinant protein was recognized by antibodies raised against regulatory protein purified from rat liver.