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Sucrose synthase genes in barley
Author(s) -
de Ilarduya Oscar Martinez,
Vicente-Carbajosa Jesus,
de la Hoz Pilar Sanchez,
Carbonero Pilar
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80087-b
Subject(s) - sucrose synthase , atp synthase , chemistry , gene , biochemistry , sucrose , invertase
A cDNA of 2,708 bp encoding type 2 sucrose synthase (Ss2) from barley has been sequenced. Similarity of this cDNA with respect to that of type 1 (Ss1) is high in the coding region (75% identical positions), but low (41% identical residues) in the 3' non‐coding region. Type‐specific non cross‐hybridizing probes for Northern blot analysis have been obtained from the 3' ends. The Ss1 type is highly expressed in developing endosperm and in roots and, at lower levels, in coleoptiles and aleurone. The Ss2 mRNA is abundant in endosperm, low in aleurone, and undetected in coleoptiles and roots.