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Trans‐activation of the murine dystrophin gene in human‐mouse hybrid myotubes
Author(s) -
Noursadeghi M.,
Walsh F.S.,
Heiman-Patterson T.,
Dickson G.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80082-6
Subject(s) - dystrophin , myogenesis , duchenne muscular dystrophy , biology , microbiology and biotechnology , gene , myocyte , cell culture , gene expression , muscular dystrophy , utrophin , genetics
Myotube cultures of the myogenic cell line, C2, produce significantly lower levels of dystrophin than primary mouse cultures. We demonstrate that expression of the C2 dystrophin gene increases 10‐fold in hybrid myotubes formed by fusion of C2 and dystrophin‐deficient human myoblasts from a Duchenne muscular dystrophy patient. These results indicate that C2 cells are deficient in endogenous gene regulatory factors which enhance dystrophin expression, and that the C2 cell line may therefore be used to identify putative trans‐acting factors involved in the regulation of dystrophin gene expression.