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Precise mapping and molecular characterization of the MFT1 gene involved in import of a fusion protein into mitochondria in Saccharomyces cerevisiae
Author(s) -
Ito Masaki,
Yasui Akira,
Komamine Atsushi
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80076-7
Subject(s) - gene , biology , fusion gene , genetics , saccharomyces cerevisiae , fusion protein , mitochondrion , mitochondrial fusion , mitochondrial dna , genome , open reading frame , microbiology and biotechnology , peptide sequence , recombinant dna
Garrett et al. [Mol. Gen. Genet. 225 (1991) 483‐491] recently reported that an Atp2‐lacZ fusion protein was transported into mitochondria in yeast, thus identifying the MFT1 (mitochondrial fusion targeting) gene as a genomic fragment which complements a mutation ( mftI ) that failed in targeting a fusion protein into mitochondria [4]. They mapped this gene to the ORF, which we have independently identified as a gene homologous to the cyc07 gene, which is expressed specifically in the S phase during the plant cell cycle. We have mapped the MFT1 gene precisely and found that this gene should correspond to the neighboring ORF, rather than the ORF they identified.