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NMR and circular dichroism studies of the lantibiotic nisin in non‐aqueous environments
Author(s) -
van den Hooven Henno W.,
Fogolari Federico,
Rollema Harry S.,
Konings Ruud N.H.,
Hilbers Cornelis W.,
van de Ven Frank J.M.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80065-3
Subject(s) - nisin , lantibiotics , aqueous solution , chemistry , circular dichroism , sodium dodecyl sulfate , micelle , membrane , biochemistry , organic chemistry , antimicrobial
The lantibiotic, nisin, which is known to interact with membranes of certain Gram‐positive bacteria, was studied in three model systems which mimic a membrane‐like environment, i.e. a mixture of trifluoroethanol and water, or micelles of sodium dodecyl sulfate or dodecylphosphocholine. The 1 H NMR spectra of nisin in the non‐aqueous environments, at 40°C and pH 3.5, have been assigned completely. The CD and NMR results indicate that the conformation of nisin in the three non‐aqueous environments differs from that in aqueous solution, and that the conformation in the two micellar systems is similar. The major conformational changes, relative to nisin in aqueous solution, occur in the N‐terminus.