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Identification of CpG island at the 5' end of murine leukemia inhibitory factor gene
Author(s) -
Kašpar Petr,
Dvořák Michal,
Bartůněk Petr
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80058-3
Subject(s) - cpg site , intron , gene , microbiology and biotechnology , leukemia inhibitory factor , exon , biology , dna , nucleotide , genetics , dna methylation , gene expression , embryonic stem cell
We identified a CpG island at the 5' end of murine leukemia inhibitory factor gene (LIF). The CpG island is 0.6 kb long and covers most of the first exon and first intron. The region is non‐methylated, its G+C content is 65% and relative frequency of CpG dinucleotide is 0.7. The block of 150 nucleotides, which is 72% conserved between murine, human, ovine and porcine genes, is a part of the CpG island. Two DNA fragments from this CpG island interact with nuclear proteins from NIH 3T3 cells. One fragment partially covers the block of conserved nucleotides. Human, ovine and porcine LIF genes also contain G+C‐ and CpG‐rich sequences in the corresponding region.