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Nucleotide sequence of cDNA encoding the fire ant venom protein Sol i II
Author(s) -
Schmidt Margit,
Walker Robert B.,
Hoffman Donald R.,
McConnell Thomas J.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80053-w
Subject(s) - complementary dna , venom , peptide sequence , nucleic acid sequence , fire ant , sequence (biology) , protein sequencing , oligonucleotide , biology , amino acid , microbiology and biotechnology , gene , biochemistry , botany , hymenoptera
For the first time the cDNA encoding a fire ant venom protein has been sequenced. Oligonucleotides were designed according to the amino acid sequence. The cDNA sequence was obtained by hybridizing these primers to mRNA and enhancement by the PCR technique. Comparison to the amino acid sequence of the venom protein shows a leader sequence 19 amino acids long.