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Evidence for binding of extrachromosomal DNA sequences to nuclear matrix proteins in multidrug‐resistant KB‐V1 cells
Author(s) -
Thiebaut Franz,
Hanauske Axel-R.,
Von Hoff Daniel D.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80052-v
Subject(s) - extrachromosomal dna , nuclear matrix , biology , microbiology and biotechnology , dna , scaffold/matrix attachment region , gene , plasmid , nuclear protein , genetics , chromatin , transcription factor , chromatin remodeling
Multidrug‐resistant KB‐V1 cells carry amplified mdrl gene sequences located in an extrachromosomal compartment (on episomes). Since episomes do not contain centromeric or telomeric sequences it is unclear whether they are able to bind to nuclear matrix proteins that may regulate episomal gene expression. Using high salt treatments followed by in situ hybridization and dot blot analyses we found evidence for direct binding of episomal DNA to nuclear matrix proteins. This binding could only be reversed after incubation with trypsin or proteinase K as determined by contour‐clamped homogeneous electric field (CHEF) electrophoresis. Our findings are consistent with the concept that circular extrachromosomal DNA may not only reintegrate into nuclear DNA but may also be subject to functional control by regulatory proteins within the nuclear matrix.