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Cleavage of human MDR 1 mRNA by a hammerhead ribozyme
Author(s) -
Kobayashi Hiroyuki,
Dorai Thambi,
Holland James F.,
Ohnuma Takao
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80039-w
Subject(s) - hammerhead ribozyme , ribozyme , cleavage (geology) , vs ribozyme , messenger rna , mammalian cpeb3 ribozyme , biology , microbiology and biotechnology , hairpin ribozyme , chemistry , rna , genetics , gene , paleontology , fracture (geology)
We designed a hammerhead ribozyme which site‐specifically cleaved the GUC sequence in codon 179 of MDR 1 mRNA. The cleavage site was 6 amino acids upstream from the drug binding site and was considered sufficiently close to the essential locus for P ‐glycoprotein function. The ribozyme cleaved the MDR 1 mRNA under physiological conditions in vitro. The cleavage was dependent on ribozyme concentration and on incubation time. Mg 2+ ion was essential for the cleavage. These results show that a potentially useful tool is at hand which may inactivate MDR 1 mRNA and revert the multidrug resistance phenotype.