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Purification and characterization of a 90 kDa protein released from human tumors and tumor cell lines
Author(s) -
Iacobelli S.,
Bucci I.,
D'Egidio M.,
Giuliani C.,
Natoli C.,
Tinari N.,
Rubistein M.,
Schlessinger J.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80037-u
Subject(s) - tumor cells , cell culture , chemistry , microbiology and biotechnology , biochemistry , biology , cancer research , genetics
A novel tumor‐associated protein, termed 90K, and recognized by mAb SP‐2 was purified from serum of breast cancer patients, ovarian cancer ascitic fluid and conditioned medium of human breast cancer cells. In these three sources, native 90K is present as a high molecular weight complex that was dissociated by SDS‐PAGE into a major band of approximately 90,000 Da. On the basis of electrophoretic mobility, buoyant density value, amino acid composition, and immunoreactivity, the 90K from the different sources appeared to be identical. NH 2 ‐tenninal amino acid sequence revealed no homology to known protein.