Premium
Effects of fatty acid oxidation on glucose utilization by isolated hepatocytes
Author(s) -
Berry Michael N.,
Phillips John W.,
Henly Debra C.,
Clark Dallas G.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80030-x
Subject(s) - glycolysis , ketone bodies , chemistry , beta oxidation , metabolism , medicine , citric acid cycle , fatty acid , biochemistry , cycling , carbohydrate metabolism , gluconeogenesis , endocrinology , biology , archaeology , history
We have studied the inhibitory action of long‐ and short‐chain fatty acids on hepatic glucose utilization in hepatocytes isolated from fasted rats. The rates of hepatic glucose phosphorylation and glycolysis were determined from the tritiated products of [2‐ 3 H] and [6‐ 3 H]glucose metabolism, respectively. The difference between these was taken as an estimate of the ‘cycling’ between glucose and glucose‐6‐phosphate. In the presence of 40 mM glucose this cycling was estimated at 0.68 μ;molmin/g wet wt. Glucose phosphorylation was unaffected during palmitate and hexanoate oxidation to ketone bodies but glycolysis was inhibited. The rate of glucose cycling was increased during this phase to 1.25 μmol/min > g. Following the complete metabolism of the fatty acids, glycolysis was reinstated and cycling rates returned to control levels. Hepatic glucose cycling appears to be an important component of the glucose/fatty acid cycle.