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Reduction in the amount of 8‐hydroxy‐2'‐deoxyguanosine in the DNA of SV40‐transformed human fibroblasts as compared with normal cells in culture
Author(s) -
Barciszewski Jan,
Rattan Suresh I.S.,
Siboska Gunhild E.,
Otzen Daniel E.,
Clark Brian F.C.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80018-p
Subject(s) - deoxyguanosine , 8 hydroxy 2' deoxyguanosine , dna , chemistry , dna damage , radical , microbiology and biotechnology , in vitro , fibroblast , biochemistry , oxidative phosphorylation , biology , dna oxidation
DNA damage due to oxidative free radicals is considered to be a major cause of ageing and age‐related diseases including cancer. Of more than 20 modifications formed in DNA by the action of hydroxyl radicals, 8‐hydroxy‐2'‐deoxyguanosine (oh 8 dG) is potentially highly mutagenic and is known to occur most frequently. Using HPLC combined with electrochemical (HPLC/EC) detection of oh 8 dG, fivefold higher levels of oh 8 dG are detected in the DNA of cultured normal human skin fibroblasts as compared with SV40‐transformed human fibroblasts MRC‐5V2. In comparison, the levels of oh 8 dG were similar in the growth medium of both types of cells. Applications of this method range from studies on the genomic stability and instability of normal and cancerous cells to the clinical and laboratory testing of toxic substances and drugs.