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Decay of Superoxide catalyzed by ferritin
Author(s) -
Bolann Bjørn J.,
Ulvik Rune J.
Publication year - 1993
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(93)80010-r
Subject(s) - ferritin , chemistry , chelation , catalysis , inorganic chemistry , biochemistry
Ferritin iron can be reduced by O .− 2 , released, and form a Fe(II)‐chelator complex. However, the thermodynamic influence of the chelator may disturb the reaction balance. We therefore excluded the chelator and measured instead the effect of ferritin on the decay of O .− 2 , monitored by direct spectrophotometry at pH 9.5. Ferritin, but not apoferritin, accelerated the decay of O − 2 . Ferritin iron was apparently the responsible agent. The effect of ferritin was maintained after several bursts of O .− 2 , and the ratio degraded O .− 2 /released Fe(II) greatly exceeded one, consistent with a catalytic reaction.