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Evidence for receptor‐linked activation of phospholipase D in rat parotid glands Stimulation by carbamylcholine, PMA and calcium
Author(s) -
Guillemain Isabelle,
Rossignol Bernard
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81533-r
Subject(s) - phosphatidylethanol , phospholipase d , endocrinology , medicine , stimulation , parotid gland , chemistry , protein kinase c , muscarinic acetylcholine receptor , calcium , phospholipase , phospholipase c , receptor , cholinergic , muscarinic acetylcholine receptor m3 , biology , biochemistry , signal transduction , phosphatidic acid , enzyme , phospholipid , dentistry , membrane
In order to test if phospholipase D (PLD) activity exists in the rat parotid gland, we took advantage of the fact that, in the presence of ethanol, PLD generates phosphatidylethanol (PEth) via a transphosphatidylation reaction. Lipid extracts of parotid acini prelabelled with [ 3 H]myristic acid were analyzed by thin layer chromatography to determine [ 3 H]phosphatidylethanol ([ 3 H]PEth) formation. Carbamylcholine (1 mM) stimulated [ 3 H]PEth formation in the presence of 2% ethanol, this effect was completely inhibited by atropine (10 μM), PMA (0.1–1 μM) and ionomycine (10 μM) also caused [ 3 H]PEth generation. We conclude that a phospholipase D activity is present in the rat parotid gland and is regulated by muscarinic cholinergic receptors. Protein kinase C and calcium could also modulate this activity. This report provides the first evidence for the existence and receptor‐linked regulation of phospholipase D in an exocrine gland, the rat parotid gland.