Premium
Uptake and metabolism of fluorescent ceramide analogs by rat oligodendrocytes in culture
Author(s) -
Giudici Maria L.,
Vos Jan P.,
Marchesini Sergio,
Van Golde Lambert M.G.,
Lopes-Cardozo Matthijs
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81529-u
Subject(s) - ceramide , sphingomyelin , biochemistry , chemistry , sphingolipid , golgi apparatus , brefeldin a , metabolism , cerebroside , oligodendrocyte , myelin , glucocerebroside , sphingosine , glycolipid , biology , cell , membrane , glucocerebrosidase , central nervous system , enzyme , endocrinology , apoptosis , receptor
We studied the metabolism of sphingolipids by oligodendrocytes derived from rat spinal cord by providing lipid vesicles with either N ‐lissamine‐rhodaminyl‐ceramide (LRh‐Cer) or N ‐(7‐nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl)‐ceramide (NBD‐Cer) to the cells cultured in a chemically‐defined medium. With both probes them the fluorescent product turned out to be sphingomyelin (SM). Most or LRh‐SM was not cell‐associated but recovered from the culture medium, probably due to back‐exchange to the lipid vesicles. The accumulation of LRh‐SM, both in the cells and in the medium, was inhibited in the presence of monensin or brefeldin A, whereas the production of NBD‐SM was much less affected by these Golgi perturbing drugs. With LRh‐Cer as substrate, LRh‐labelled fatty acid (FA), galactosyl‐ and sulfogalactosyl‐ceramides (GalCer and SGalCer) were also formed. NBD‐Cer, however, was metabolized to glucosylecramide (GlcCer) and GalCer but not to SGalCer or NBD‐FA. These data demonstrate that chemical modifications of ceramide alter its metabolism in oligodendrocytes and that the metabolites of LRh‐Cer reflect the glycolipid composition of myelin more closely than those of NBD‐Cer.