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Identification of a MAP kinase kinase kinase in phaeochromocytoma (PC12) cells
Author(s) -
Gomez Nestor,
Traverse Sarah,
Cohen Philip
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81527-s
Subject(s) - cyclin dependent kinase 9 , mitogen activated protein kinase kinase , map2k7 , kinase , identification (biology) , cyclin dependent kinase 2 , map kinase kinase kinase , ask1 , cyclin dependent kinase 4 , protein kinase r , protein kinase a , chemistry , microbiology and biotechnology , biology , botany
A MAP kinase kinase kinase (MAPKKK) was identified in phaeochromocytoma (PC12) cells which reactivated homogeneous MAP kinase kinase (MAPKK) from rabbit skeletal muscle that had been inactivated by incubation with protein phosphatase 2A. Reactivation was accompanied by stoichiometric phosphorylation of MAPKK on a serine residue(s). Following stimulation of PC12 cells with nerve growth factor and chromatography of the extracts on Mono Q, MAP kinase and MAPKK were detected as active phosphorylated enzymes, whereas MAPKKK was inactive and only activated after prolonged storage at 4°C. The results suggest that the activation of MAPKKK by growth factors is likely to occur by a non‐covalent mechanism.