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Secondary structure of human granulocyte colony‐stimulating factor derived from NMR spectroscopy
Author(s) -
Zink Thomas,
Ross Alfred,
Ambrosius Dorothee,
Rudolph Rainer,
Holak Tad A.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81521-m
Subject(s) - protein secondary structure , recombinant dna , granulocyte colony stimulating factor , nuclear magnetic resonance spectroscopy , chemistry , granulocyte , residue (chemistry) , protein structure , stereochemistry , biochemistry , biology , immunology , gene , genetics , chemotherapy
Recombinant 13 N‐. 13 C‐labeled human granulocyte colony‐stimulating factor (rh‐metG‐CSF) has been studied by 2D and 3D NMR using uniformly labeled protein as well is residue‐specific 13 N‐labeled samples. Assignment of the 1 H, 15 N backbone, and 60% 1 H sidechain resonances has enabled the determination of the secondary structure of the protein. The secondary structure is dominated by α‐helical regions with four stretches of helices between residues 11–41, 71–95, 102–124 and 144–170.