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Interdependence of respiratory NO reduction and nitrite reduction revealed by mutagenesis of nir Q, a novel gene in the denitrification gene cluster of Pseudomonas stutzeri
Author(s) -
Jüngst Angelika,
Zumft Walter G.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81495-8
Subject(s) - nitrite reductase , pseudomonas stutzeri , biochemistry , mutagenesis , gene cluster , nitrite , gene , chemistry , biology , microbiology and biotechnology , enzyme , nitrate , genetics , nitrate reductase , mutation , organic chemistry , bacteria
An open reading frame, designated nir Q, was identified upstream or nir S, the structural gene for the respiratory nitrite reductase or Pseudomonas stutzeri ZoBell. Its derived gene product (275 amino acids, M r = 30.544) shows similarity to the NtrC protein family or transcriptional activators. Deletion—replacement mutagenesis of the nir Q gene resulted in the simultaneous loss of nitrite reduction and NO reduction in vivo. However, both reductases were still synthesized, with only nitrite reductase being active in vitro. NO reductase was overproduced by a factor of about 2. Our results indicate that the systems for nitrite reduction and NO reduction are functionally coupled.