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Investigation of the neighbour relationships between photosystem II polypeptides in the two types of isolated reaction centres (D1/D2/cyt b 559 and CP47/D1/D2/cyt b 559 complexes)
Author(s) -
Moskalenko Andrey A.,
Barbato Roberto,
Giacometti Giorgio M.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81487-7
Subject(s) - chemistry , photosystem ii , bifunctional , alpha (finance) , biochemistry , photosynthesis , catalysis , medicine , construct validity , nursing , patient satisfaction
The nearest neighbour relationships within the D1/D2/cyt b 559 complex (PSIIRC) and the CP47/D1/D2/cyt b 559 complex (RC‐CP47) were investgated by using different length bifunctional crosslinking agents. The crosslinking products were identified by immunoblotting with polyclonal antibodies and by two‐dimensional gel electrophoresis. Seven products (CP47/D2, D1/D2/α, D1/D2, D2/α, D1/α, α/αa, α/β) have been revealed in both complexes. The crosslinking of both complexes does not increase their photostability. The photocrosslinking products (D1/α and D2/α) appeared under illumination of complexes with light of high intensity.