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Two subforms of eukaryotic topoisomerase I Purification and structure‐function relationships
Author(s) -
Kudinov Alexei R.,
Bronstein Igor B.,
Gabibov Alexander G.,
Gololobov Gennady V.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81486-6
Subject(s) - topoisomerase , chemistry , function (biology) , computational biology , biochemistry , microbiology and biotechnology , biology , dna
A new method for isolation of eukaryotic topoisomerase 1 from calf thymus and from Jurkat‐1 cells using HPLC has been developed. The method allows quantitative purification of high molecular weight topo I and of two low molecular weight fractions differing by their isoelectric points. It has been suggested that these fractions be characterized as two subforms of the enzyme possessing structural and functional differences. The differences in their specific activities, sensitivity to camptothecin and in their proteolytic digestion maps have been demonstrated for the two enzymes.