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Receptor mediated mineralocorticoid action in alga cell mutants
Author(s) -
Mirshahi M.,
Mirshahi A.,
Nato A.,
Agarwal M.K.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81479-6
Subject(s) - steroid , receptor , biology , mutant , microbiology and biotechnology , mineralocorticoid receptor , steroid hormone , cell division , population , aldosterone , hormone , biochemistry , cell , gene , genetics , demography , sociology
The multiplication of Chlamydomonas cells can be arrested by the spirolactone derivative RU 26752 and this is fully reversible by the natural hormone aldosterone. Continuous growth in the presence of RU 26752 led to the isolation of a population subsequently resistant to the action of mineralocortoid analogues, due possibly to the selection of mutant cells. Immunophotochemical evidence is provided for a 52 kDa protein that possesses functional steroid and DNA binding domains. Alga cells therefore appear to respond to steroid hormones in a manner similar to the mammalian systems, possibly via a receptor that may represent a pygmy ancestor of the latter day steroid receptor superfamily.