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Inhibition of passive cutaneous anaphylaxis by synthetic human immunoglobulin E peptide fragments
Author(s) -
Nio Noriki,
Seguro Katsuya,
Ariyoshi Yasuo,
Ishii Katsumi,
Nakamara Hideo
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81477-4
Subject(s) - peptide , immunoglobulin e , chemistry , anaphylaxis , antibody , in vivo , amino acid , peptide sequence , biochemistry , microbiology and biotechnology , allergy , immunology , biology , gene
In an attempt to determine the regions responsible for type 1 immediate hypersensitivity, a total of 42 peptide fragments, which cover the CH3–CH4 domains in human immunoglobulin E (IgE), were chemically synthesized. Several peptide fragments located in the amino acid sequences Ala 329 —Thr 357 and Arg 119 —Ala 463 , inhibited passive cutaneous anaphylaxis (PCA) in vivo. In order to pinpoint the sites responsible for the inhibition of the PCA reaction, various rragment peptides in these two regions were synthesized. As a result, residues Pro 343 —Leu 348 , Pro 426 —Thr 433 , and Ser 456 —Thr 401 were suggested to be involved in type I immediate hypersensitivity.