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A DNA fragment homologous to F 1 ‐ATPase β subunit was amplified from genomic DNA of Methanosarcina barkeri Indication of an archaebacterial F‐type ATPase
Author(s) -
Sumi Masato,
Sato Masa H.,
Denda Kimitoshi,
Date Takayasu,
Yoshida Masasuke
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81472-x
Subject(s) - methanosarcina barkeri , genomic dna , atpase , biology , microbiology and biotechnology , dna , gene , oligonucleotide , nucleic acid sequence , protein subunit , biochemistry , genetics , enzyme , methanogenesis , bacteria
A 490 bp DNA fragment was amplified from Methanosarcina barkeri genomic DNA by the polymerase chain reaction (PCR) using oligonucleotide primers designed based on conserved amino acid sequences of the F 1 ‐ATPase β subunits. The amino acid sequence deduccd from the DNA sequence of this fragment was highly homologous to a portion of the F 1 ‐ATPase β subunit. This indicates that this archaebacterium has a gene of F‐type ATPase in addition to a gene of V‐type ATPase.