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‘All‐or‐none’ mechanism of the molten globule unfolding
Author(s) -
Uversky V.N.,
Semisotnov G.V.,
Pain R.H.,
Ptitsyn O.B.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81468-2
Subject(s) - molten globule , chemistry , crystallography , carbonic anhydrase , molten salt , mechanism (biology) , thermodynamics , biophysics , biochemistry , enzyme , inorganic chemistry , biology , circular dichroism , physics , quantum mechanics
The Gdm‐HCl‐induced unfolding of bovine carbonic anhydrase B and S . aureus β‐lactamase was studied at 4°C by a variety of methods. With the use of FPLC it has been shown that within the transition from the molten globule to the unfolded state the distribution function of molecular dimensions is bimodal . This means that equilibrium intermediates between the molten globule and the unfolded states are absent, i.e. the molten globule unfolding follows the ‘all‐or‐none’ mechanism.