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Nitric oxide‐releasing compounds inhibit Dictyostelium discoideum aggregation without altering cGMP production
Author(s) -
Tao Yongping,
Howlett Allyn,
Klein Claudette
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81459-y
Subject(s) - dictyostelium discoideum , sodium nitroprusside , soluble guanylyl cyclase , nitric oxide , chemistry , microbiology and biotechnology , biochemistry , guanylate cyclase , biology , gene , organic chemistry
The effects of nitric oxide‐releasing compounds on Dictyostelium discoideum cell development and guanylyl cyclase activity were studied. The addition of SNP (sodium nitroprusside) or SIN‐1 (3‐morpholino‐syndnonimine) to starved cells inhibited their differentiation and aggregation in a concentration‐dependent manner. In contrast to mammalian systems, SNP did not significantly affect guanylyl cyclase activity in cell lysates of D. discoideum nor did it stimulate cGMP production in intact cells. The results suggest that the inhibitory effects of NO on D. discoideum cell aggregation are through a mechanism independent of an effect on guanylyl cyclase activity.