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Human peripheral blood lymphocytes express D5 dopamine receptor gene and transcribe the two pseudogenes
Author(s) -
Takahashi Nobuyuki,
Nagai Yoshitaka,
Ueno Satoshi,
Saeki Yoshinaga,
Yanagihara Takehiko
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81452-r
Subject(s) - pseudogene , gene , microbiology and biotechnology , biology , complementary dna , receptor , dopamine receptor , dopaminergic , reverse transcriptase , reverse transcription polymerase chain reaction , dopamine , messenger rna , polymerase chain reaction , genetics , endocrinology , genome
Sequential reverse transcription and polymerase chain reaction (RT‐PCR) of the mRNA were used to investigate the expression of dopamine receptors in human peripheral blood lymphocytes. The RT‐PCR products contained three types of sequences, each corresponding to those of the D5 dopamine receptor gene and the two related pseudogenes. The lymphocyte cDNA library also contained the clones encoding parts of the three genes. Binding profiles of dopaminergic ligands to the lymphocytes were similar to those for the native neuronal membranes.