Premium
Structure of UvrABC excinuclease—UV‐damaged DNA complexes studied by flow linear dichroism DNA curved by UvrB and UvrC
Author(s) -
Takahashi Masayuki,
Bertrand-Burggraf Elisabeth,
Fuchs Robert P.P.,
Nordén Bengt
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81448-u
Subject(s) - linear dichroism , dna , biophysics , circular dichroism , dichroism , chemistry , nucleotide excision repair , helix (gastropod) , dna damage , optics , crystallography , biochemistry , biology , physics , ecology , snail
The interaction between UvrABC excinuclease from Escherichia coli and ultraviolet light‐(UV) damaged DNA was studied by flow linear dichroism. The dichroism signal from DNA was drastically decreased in intensity upon incubation with UvrA and UvrB or whole enzyme in the presence off effector ATP. The change was specific for UV‐damaged DNA, and a concluded suppressed DNA orientation suggests the wrapping of DNA around the protein. The incubation with the UvrC subunit alone also somewhat reduces the signal, however, in this case the change was smaller and not specific for UV‐damaged DNA. The structural modification of DNA, promoted by the (UvrA 2 ‐UvrB) complex probably facilitates or stabilizes the interaction of the UvrC subunit with DNA for the excision.