Premium
ATP‐sensitive K + ‐channels in the human adult ventricular cardiomyocytes membrane
Author(s) -
Babenko Andrew P.,
Samoilov Vladimir O.,
Kazantseva Svetlana T.,
Shevchenko Yuri L.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81432-l
Subject(s) - sarcolemma , conductance , membrane potential , biophysics , membrane , chemistry , patch clamp , ion channel , physics , biochemistry , biology , condensed matter physics , receptor
Using the patch‐clamp method in 27 different inside‐out patches it was shown for the first time that they defined the basic parameters of the functioning of single ATP‐sensitive K + channels in the human adult ventricular cardiomyocytes membrane. At [K + ] o = 140 mM single channel conductance (over the linear part of the I—V relation) reaches 100 pS. The possibility of the existence of these channels' conductance sublevels as well as the cluster character of their localization in sarcolemma is shown. The channel activity demonstrates an obvious run‐down with τ at about a minute. The analysed channels possess one open and two closed states.