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Codon—anticodon pairing A model for interacting codon—anticodon duplexes located at the ribosomal A‐ and P‐sites
Author(s) -
Lim Valery I.,
Venclovas Česlovas
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81429-p
Subject(s) - transfer rna , ribosomal rna , pairing , codon usage bias , start codon , ribosome , genetics , chemistry , biology , physics , base sequence , rna , gene , genome , superconductivity , quantum mechanics
The interaction between two codon—anticodon duplexes of the ribosomal A‐ and P‐site‐bound tRNAs is the key feature of the proposed model. This interaction prohibits non‐canonical base pairing at the first and second positions of the codon and controls base pairing at the third position (wobbling rules ensuing from the model are in good accord with those generated from experiments). The model is capable of predicting codon context effects. It follows from the model that modifications of the first anticodon residue of the P‐site tRNA can affect the stability of the A‐site duplex, and that the translation of a DNA single chain analogue of mRNA should be accompanied by non‐canonical base pairing at all three positions of the codon. These predictions of the model can be subjected to experimental tests.