Premium
Localization of myosin IIB at the leading edge of growth cones from rat dorsal root ganglionic cells
Author(s) -
Cheng Toni P.O.,
Murakami Noriko,
Elzinga Marshall
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81374-u
Subject(s) - myosin , neurite , actin , microbiology and biotechnology , growth cone , motility , antibody , biology , dorsal root ganglion , chemistry , anatomy , dorsum , biochemistry , immunology , in vitro , axon
Primary cultures of rat dorsal root ganglionic (DRG) cells were stained With isoform‐specific antibodies against non‐muscle myosin II. Antibodies against the brain type myosin (MIIB) stained the peripheries of growth cones and non‐neuronal cells. Double staining of the cells with the anti‐myosin antibodies and rhodamine‐phalloidin or anti‐actin antibodies indicated that MIIB co‐exists, with F‐actin, at the leading edge. Antibodies against platelet myosin stained neither leading edges nor neurites, but stained the cell bodies of neurons and the stress fibers of non‐neuronal cells. These results suggest that MIIB functions in the motility of the leading edge of DRG cells.