Premium
Cloning and sequencing of the cDNA encoding rice elongation factor 1β′
Author(s) -
Matsumoto Shogo,
Oizumi Naoto,
Taira Hideharu,
Ejiri Shin-ichiro
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81363-q
Subject(s) - cloning (programming) , complementary dna , elongation factor , genetics , encoding (memory) , elongation , biology , eukaryotic translation elongation factor 1 alpha 1 , molecular cloning , gene , computational biology , computer science , rna , ribosome , materials science , neuroscience , ultimate tensile strength , metallurgy , programming language
A cDNA clone coding for rice elongation factor 1β′(EF‐1β′) was isolated from a rice anther cDNA library. The clone, named RB′, was 980 bp long and contained a single open reading frame coding for 223 amino acids; the first 31 amino acids, except for the first methionine, which is absent in the mature protein, are identical to those of the purified protein determined with a protein sequencer. The amino acid sequence of rice EF‐1β′ shows homology to the C‐terminal half of Artemia salina EF‐1β (59%) and human EF‐1β (63%), but might not have a phosphorylation site for casein kinase II which has been conserved in Artemia salina EF‐1β and EF‐σ, human EF‐1β and silkworm EF‐1β′.