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Novel ion channels in the protists, Mougeotia and Saprolegnia , using sub‐gigaseals
Author(s) -
Lew Roger R.,
Garrill Ashley,
Covic Lidija,
Heath I.Brent,
Serlin Bruce S.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81336-k
Subject(s) - saprolegnia , oomycete , protoplast , pipette , biology , patch clamp , ion channel , biophysics , botany , chemistry , microbiology and biotechnology , biochemistry , fish <actinopterygii> , fishery , receptor , pathogen
Protoplasts of the filamentous alga, Mougeotia , and the filamentous fungal oomycete, Saprolegnia ferax , exhibit two K + ion channels (2–6 pA) using the patch‐clamp technique when the seals are less than 1 GΩ (about 100 MΩ). The membrane potential of the protoplasts was near 0 mV as measured intracellularly with double‐barreled micropipettes; thus, inward K + flux is due solely to concentration differences. Although conductances are in the range expected for K + channels, the activity at 0 mV is not seen in other organisms under gigaseal conditions. This paper draws attention to the usefulness of this subsidiary patch‐clamp technique and the novel characteristics of ion channels in Mougeotia and Saprolegnia .