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Single cell observation of ligand‐induced desensitization of B‐cell membrane immunoglobulin‐mediated calcium signals
Author(s) -
Yamada Hiroshi,
Mizuguchi Junichiro,
Noji Masahide,
Nakanishi Mamoru
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81329-k
Subject(s) - desensitization (medicine) , receptor , chemistry , fluorescence microscope , antibody , phorbol , calcium , cell membrane , microbiology and biotechnology , membrane , biology , biochemistry , protein kinase c , immunology , fluorescence , signal transduction , physics , organic chemistry , quantum mechanics
Using a digital imaging fluorescence microscope we have observed the membrane immunoglobulin (mIg)‐induced desensitization of calcium signals in individual BAL17 B lymphoma cells which express two kinds of antigen receptors, mIgM and mIgD. The mIgD‐mediated desensitization was partly abrogated by pretreating the cells with phorbol 12‐myristate 13‐acetate (PMA) for 24 h, however, the mIgM‐mediated one was not affected by the pretreatment. This supports the idea that at least two mechanisms are operative for mIg‐induced desensitization in B cells.