Premium
De novo synthesis and desaturation of fatty acids at the mitochondrial acyl‐carrier protein, a subunit of NADH:ubiquinone oxidoreductase in Neurospora crassa
Author(s) -
Zensen Ralf,
Husmann Heribert,
Schneider Regina,
Peine Thorsten,
Weiss Hanns
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81324-f
Subject(s) - neurospora crassa , biochemistry , oxidoreductase , acyl carrier protein , biology , protein subunit , fatty acid synthesis , cytosol , cardiolipin , neurospora , fatty acid , biosynthesis , enzyme , mutant , phospholipid , membrane , gene
We have cultivated the cel mutant of Neurospora crassa defective in cytosolic fatty acid synthesis with [2‐ 14 C]malonate and found radioactivity covalently attached to the mitochondrial acyl‐carrier protein (ACP), a subunit of the respiratory chain NADH:ubiquinone oxidoreductase. We purified the ACP by reverse‐phase HPLC: the bound acyl groups were trans‐esterified to methylesters and analyzed by gas chromatography. The saturated C 0 to C 18 fatty acids and oleic acid were detected. De novo synthesis and desaturation of fatty acids at the ACP subunit of NADH:ubiquinone oxidoreductase and use of the products of this mitnchondrial synthetic pathway for cardiolipin synthesis is discussed.