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Developmental expression of voltage‐sensitive K + channels in mouse skeletal muscle and C 2 C 12 cells
Author(s) -
Lesage Florian,
Attali Bernard,
Lazdunski Michel,
Barhanin Jacques
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81320-l
Subject(s) - skeletal muscle , microbiology and biotechnology , biology , gene expression , shaker , messenger rna , myocyte , western blot , gene , chemistry , genetics , anatomy , physics , quantum mechanics , vibration
The developmental expression of voltage‐sensitive K + channels was analyzed by Northern blot in mouse skeletal muscle. Of nine Shaker ‐like genes studied, eight are expressed in this mammalian muscle. Their expression is differentially regulated during development. The mouse cell line C 2 C 12 has been used to study expression of voltage‐sensitive K + channels during in vitro myotube differentiation. Different voltage‐sensitive K + channel messages are also expressed in these cells which display a pattern of expression depending upon the differentiation stage. The message for the very peculiar K + channel or IsK type could only be detected by polymerase chain reaction on skeletal muscle mRNA.