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The inverted hexagonal phase is more sensitive to hydroperoxidation than the multilamellar phase in phosphatidylcholine and phosphatidylethanolamine aqueous dispersions
Author(s) -
Ye Wang Jin,
Miyazawa Teruo,
Fujimoto Kenshiro,
Wang ZhengYu,
Nozawa Tsunenori
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81307-8
Subject(s) - hexagonal phase , phosphatidylethanolamine , phospholipid , bilayer , chemistry , phosphatidylcholine , phase (matter) , lamellar phase , lipid bilayer , aqueous solution , aqueous two phase system , chromatography , analytical chemistry (journal) , crystallography , organic chemistry , membrane , biochemistry
The effect of phase behaviour (hexagonal II phase and lamellar phase) on the peroxidation of membrane phospholipids has been investigated in dilinoleoyl phosphatidylcholine (DLPC)/dilinoleoyl phosphatidylethanolamine (DLPE) aqueous dispersions. Peroxidation was initiated with a water‐soluble radical inducer 2,2′‐azobis (2‐amidino‐propane) dihydrochloride (AAPN). The phospholipid morphology was monitored by 31 P‐nuclear magnetic resonance (NMR). Phospholipid hydroperoxides (PCOOH and PEOOH) were determined by chemiluminescence high‐performance liquid chromatography (CL—HPLC). In pH‐induced phase transition systems, DLPE in the bilayer state was much less oxidized than in the hexagonal II state. In composition‐induced phase transition systems, the formation of total hydroperoxides and the consumption of α‐tocopherol in the hexagonal II phase were greater than in the bilayer phase. These data suggest that the hexagonal II phase is more sensitive to hydroperoxidation than the bilayer phase in phospholipid aqueous dispersions.