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Interleukin‐1β induces the expression of an isoform of nitric oxide synthase in insulin‐producing cells, which is similar to that observed in activated macrophages
Author(s) -
Eizirik Décio L.,
Cagliero Enrico,
Björklund Andres,
Welsh Nils
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81285-t
Subject(s) - nitric oxide synthase , nitric oxide , insulin , gene isoform , chemistry , cytotoxic t cell , arginine , interleukin , omega n methylarginine , endocrinology , microbiology and biotechnology , medicine , biology , in vitro , biochemistry , cytokine , immunology , gene , amino acid
The suppressive and cytotoxic effects of interleukin‐1β(IL‐β) on rodent insulin‐producing cells observed in vitro are probably mediated through formation or nitric oxide (NO). In this study we demonstrate that IL‐1‐induced NO formation in isolated rat islets and insulin‐producing HIT cells is more sensitive to inhibition by N G ‐monomethyl‐ l ‐arginine than to inhibition by N G ‐nitro‐ l ‐arginine, thus suggesting that IL‐1‐exposed insulin producing cells express an isoform of nitric oxide synthase similar to that present in activated macrophages, Furthermore, IL‐1β markedly increased the mRNA levels or the inducible microphage form of nitric oxide synthase in HIT cells.