Premium
Reversal of oxidative phosphorylation in submitochondrial particles using glucose 6‐phosphate and hexokinase as an ATP regenerating system
Author(s) -
de Meis Leopoldo,
Grieco Maria Angela B.,
Galina Antonio
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81273-o
Subject(s) - hexokinase , oxidative phosphorylation , submitochondrial particle , glucose 6 phosphate , chemistry , phosphate , biochemistry , phosphorylation , adenosine triphosphate , glycolysis , uncoupling agents , atp synthase , mitochondrion , enzyme
The different reaction measured with the use of glucose‐6‐phosphate and hexokinase were inhibited by glucose concentrations varying from 0.2 to 2 mM. Glucose shifts be equilibrium of the reaction towards glucose‐6‐phosphate formation thus leading to a decrease of the ATP concentration in the medium.