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Over‐expression of human phospholipase C‐γ2 enhances platelet‐derived growth factor‐induced mobilization of intracellular Ca 2+ and the release of arachidonic acid and prostaglandins in NIH 3T3 fibroblasts
Author(s) -
Totzke Frank,
Hug Hubert,
Fitzke Edith,
Marmé Dieter,
Dieter Peter
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81258-n
Subject(s) - arachidonic acid , phospholipase c , phospholipase , phospholipase a2 , growth factor , intracellular , chemistry , prostaglandin , platelet derived growth factor receptor , prostaglandin e2 , phospholipase d , endocrinology , biology , biochemistry , signal transduction , enzyme , receptor
Over‐expression of human phospholipase C‐γ2 in murine NIH 3T3 fibroblasis has been shown to result in an increased platelet‐derived growth factor‐mediated formation of inositol phosphates. Here we show that phospholipase C‐γ2 over‐expression is associated with an increased platelet‐derived growth factor‐mediated release of arachidonic acid, prostaglandin E 2 , 6‐keto prostaglandin F 10 and prostaglandin F 20 . The phorbol ester, calcium ionophore‐ and fluoride‐induced release of arachidonate and its metabolites is not affected by phospholipase C‐γ2 over‐expression. Over‐expression of phospholipase C‐γ2 is also associated with an enhancement of platelet‐derived growth factor‐induced change in intracellular Ca 2+ . These results demonstrate that stimulation of recombinant human phospholipase C‐γ2 induces a change in the intracellular Ca 2+ concentration, a release of arachidonic acid and formation of prostaglandins in NIH 3T3 cells. In control cells platelet‐derived growth factor‐induced activation of arachidonic acid cascade is rate‐limited by the endogenous phospholipase C.