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Transforming growth factors type‐β and dexamethasone attenuate group II phospholipase A 2 gene expression by interleukin‐1 and forskolin in rat mesangial cells
Author(s) -
Mühl Heiko,
Geiger Thomas,
Pignat Werner,
Märki Fritz,
van den Bosch Henk,
Cerletti Nico,
Cox David,
McMaster Garry,
Vosbeck Klaus,
Pfeilschifter Josef
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81245-h
Subject(s) - forskolin , medicine , endocrinology , dexamethasone , transforming growth factor , glucocorticoid , secretion , messenger rna , interleukin , gene expression , phospholipase a , biology , chemistry , phospholipase a2 , cytokine , gene , enzyme , biochemistry , stimulation
Treatment of rat mesangial cells with interleukin‐1β(IL‐1β) and forskolin induced, in a synergistic fashion,the expression of group II phospholipase A 2 (PLA 2 ) mRNA, with subsequent increased synthesis and secretion of PLA 2 . In contrast, interleukin‐6 did not increase PLA 2 mRNA levels of PLA 2 activity. Transforming growth factor (TGF) β 1 , TGFβ 2 and TGFβ 3 equipotently attenuated the IL‐1β‐ and forskolin‐induced elevation of PLA 2 mRNA, as well as PLA 2 synthesis and secretion. The glucocorticoid dexamethasone only partially suppressed the IL‐1β‐ and forskolin‐induced elevation of PLA 2 mRNA, but totally inhibited PLA 2 synthesis and secretion.