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Ganglio‐ N ‐tetraosylceramide (GA1) of bovine and human brain Molecular characterization and presence in myelin
Author(s) -
Dasgupta S.,
van Halbeek H.,
Hogan E.L.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81234-d
Subject(s) - antiserum , chemistry , exoglycosidase , hydrolysis , immunostaining , myelin , heteronuclear molecule , chromatography , biochemistry , oligosaccharide , human brain , nuclear magnetic resonance spectroscopy , stereochemistry , biology , glycoprotein , immunohistochemistry , antibody , immunology , central nervous system , neuroscience , glycan
During our studies of bovine brain neutral glycosphingolipids (Ngsl's), we have purified a compound that co‐migrates on thin‐layer chromatogram with standard GA1 (purified by acid hydrolysis of GM1) and close to penta‐ (nLc 5 Cer) glycosylceramide from bovine erythrocytes. The structure of the purified Ngsl from brain has been established by permethylation and by stepwise exoglycosidase hydrolysis. 600 MHz 1 H NMR spectroscopy of the oligosaccharide obtained from the Ngsl after endoglycoceramidase hydrolysis confirms the structure as ganglio‐ N ‐tetraosylceramide (GgOse 4 Cer or GA1) as Ga1β1→3Ga1NAcβ1‐4Galβ1→4G1cβ1→1Cer. We have identified GA1 in bovine, rat and human brain and myelin by TLC‐immunostaining with monospecific anti‐GA1 antiserum.