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Incorporation of N ‐acyl‐2‐amino‐2‐deoxy‐hexoses into glycosphingolipids of the pheochromocytoma cell line PC 12
Author(s) -
Kayser Holger,
Geilen Christoph C.,
Paul Christine,
Zeitler Reinhard,
Reutter Werner
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81233-c
Subject(s) - chemistry , pheochromocytoma , biochemistry , cell culture , stereochemistry , endocrinology , biology , genetics
The synthesis of N ‐acyl‐2‐amino‐2‐deoxy‐hexoses, their metabolism and their incorporation into glycosphingolipids of rat pheochromocytoma cell line PC 12 were investigated. The data indicate that in PC 12 cells the N ‐acyl‐2‐amino‐2‐deoxy‐hexoses, N ‐propanoyl‐ d ‐glucosamine and N ‐butanoyl‐ d ‐glucosamine are metabolized to the corresponding phosphates, and that N ‐propanoyl‐ d ‐glucosamine is also metabolized to N ‐propanoyl neuraminic acid. Using variously radiolabelled N ‐acyl‐2‐amino‐2‐deoxy‐hexoses, their incorporation into glycosphingolipids was shown.