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ATP‐activated Ca 2+ ‐permeable channels in rat peritoneal macrophages
Author(s) -
Naumov Alexander P.,
Kuryshev Yuri A.,
Kaznacheyeva Elena V.,
Mozhayeva Gali.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81210-d
Subject(s) - gtp' , extracellular , biophysics , chemistry , intracellular , patch clamp , membrane , sodium , receptor , biochemistry , biology , enzyme , organic chemistry
The patch‐clamp technique was used to study mechanisms of ATP‐induced Ca 2+ influx in rat peritoneal macrophages. The experiments on whole‐cell and patch membranes have shown that extracellular ATP activates channels permeable to di‐ and monovalent inorganic cations. Ratios of unitary channel conductances in 105 mM Ca 2+ , Sr 2+ , Mn 2+ , Ba 2+ and normal sodium solutions were 1.0, 0.95, 0.75, 0.55 and 0.85, respectively. The channels could open in the presence of non‐hydrolyzable GTP analogues in artificial intracellular solution. The data are consistent with the hypothesis that a GTP‐binding protein is involved in receptor‐to‐channel coupling.