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Mode of action of the Spiroplasma CpG methylase M.SssI
Author(s) -
Renbaum Paul,
Razin Aharon
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81201-v
Subject(s) - processivity , dna , spiroplasma , cpg site , biology , cytosine , methyltransferase , circular bacterial chromosome , biochemistry , 5 methylcytosine , dna polymerase , dna methylation , microbiology and biotechnology , genetics , methylation , mollicutes , gene , bacteria , gene expression
The cytosine DNA methylase from the wall‐less prokaryote, Spiroplasma strain MQ1 (M.SssI) methylates completely and exclusively CPG‐containing sequences, thus showing sequence specificity which is similar to that of mammalian DNA methylases. M.SssI is shown here to methylate duplex DNA processively as judged by kinetic analysis of methylated intermediates. The cytosine DNA methylases, M.Hpall and M.Hhal, from other prokaryotic organisms, appear to methylate in a non‐processive manner or with a very low degree of processivity. The Spiroplasma enzyme interacts with duplex DNA irrespective to the presence of CpG sequences in the substrate DNA. The enzyme proceeds along a CpG‐containing DNA substrate molecule methylating one strand of DNA at a time.