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Identification of nuclear factors which interact with the 5′ flanking region of the EF‐1αO gene in Xenopus laevis
Author(s) -
Olesen Ole F.,
Frydenberg Jane
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81192-o
Subject(s) - xenopus , gene , biology , 5' flanking region , microbiology and biotechnology , promoter , regulatory sequence , nuclear protein , dna , genetics , homology (biology) , oocyte , regulation of gene expression , gene expression , transcription factor , embryo
The EF‐1αO gene of Xenopus laevis is a stage‐specific gene, being transcribed in oogonia and oocytes, but not in postmeiotic germ cells and terminally differentiated cells. We found that two trans ‐acting factors from oocyte nuclear extract are able to interact with a DNA sequence in the 5′‐upstream region of the EF‐1αO gene. Methylation interference experiments suggested that the two factors recognised the same DNA element. Gel retardation assays indicated that part of the protein binding site could be confined to a 21 bp sequence, located between −51 and −72, relative to the cap site. Interestingly, this region shares great homology to a negative regulatory segment in the promoter of the TFIIIA gene, another developmentally regulated gene.