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Osteoclasts express high levels of p60 c‐ src , preferentially on ruffled border membranes
Author(s) -
Tanaka Sakae,
Takahashi Naoyuki,
Udagawa Nobuyuki,
Sasaki Takahisa,
Fukui Yasuhisa,
Kurokawa Takahide,
Suda Tatsuo
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81190-w
Subject(s) - multinucleate , rous sarcoma virus , podosome , osteoclast , biology , proto oncogene tyrosine protein kinase src , vacuole , bone resorption , microbiology and biotechnology , in vitro , biochemistry , cytoplasm , kinase , cell , endocrinology , cytoskeleton , gene
Expression of p60 c‐ src , the normal cellular counterpart of the transforming protein of Rous sarcoma virus (RSV), p60 c‐ src , was examined in mouse and rat authentic osteoclasts and mouse osteoclast‐like multinucleated cells (MNCs) formed in vitro. In co‐cultures of mouse osteoblastic cells and spleen cells, the expression of p60 c‐ src strikingly increased on day 5 in parallel with the appearance of MNCs in the presence of 1α,25‐dihydroxyvitamin D 3 (1α,25(OH) 2 D 3 ). Immunohistochemical examination confirmed the high level expression of p60 c‐ src in both mouse authentic osteoclasts and MNCs. Electron microscopic examination revealed that p60 c‐ src was primarily localized on ruffled border membranes and vacuoles, but not on the clear zone in rat authentic osteoclasts. These results suggest that p60 c‐src is important in osteoclastic bone resorption.