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Interaction of nitrogen dioxide with human plasma Antioxidant depletion and oxidative damage
Author(s) -
Halliwell Barry,
Hu Miao-Lin,
Louie Samuel,
Duvall Timothy R.,
Tarkington Brian K.,
Motchnik Paul,
Cross Carroll E.
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81185-o
Subject(s) - lipid peroxidation , uric acid , chemistry , ascorbic acid , antioxidant , biochemistry , nitric oxide , bilirubin , in vivo , vitamin e , medicine , food science , organic chemistry , biology , microbiology and biotechnology
Nitrogen dioxide (NO* 2 ) is often present in inhaled air and may be generated in vivo from nitric oxide. Exposure of human blood plasma to NO* 2 caused rapid losses of ascorbic acid, uric acid and protein thiol groups, as well as lipid peroxidation and depletions of α‐tocopherol, bilirubin and ubiquinol‐10. No increase in protein carbonyls was detected. Supplementation of plasma with ascorbate decreased the rates of lipid peroxidation. α‐tocopherol depletion and loss of uric acid. Uric acid supplementation decreased rates of lipid peroxidation but not the loss of α‐tecopherol. We conclude that ascorbic acid, protein ‐SH groups, uric acid and α‐tocopherol may be important agents protecting against NO* 2 in vivo. If these antioxidants are depleted, peroxidation of lipids occurs and might contribute to the toxicity of NO* 2 .