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m ‐Iodobenzylguanidine increases the mitochondrial Ca 2+ pool in isolated hepatocytes
Author(s) -
Juedes Marlene J.,
Kass George E.N.,
Orrenius Sten
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81179-p
Subject(s) - cytosol , endoplasmic reticulum , mitochondrion , chemistry , intracellular , incubation , biophysics , biochemistry , biology , enzyme
The incubation of isolated hepatocytes with the inhibitor of protein mono ADP‐ribosylation, m ‐iodobenzylguanidine (MIBG), resulted in an increase in the size of the mitochondrial Ca 2+ pool, without alteration of the non‐mitochondrial Ca 2+ store(s). This increase was abolished when the cytosolic free Ca 2+ concentration ([Ca 2+ ] i ) was buffered by prior loading of the cells with fluo 3. Elevating [Ca 2+ ] i by releasing the endoplasmic reticular Ca 2+ store with 2,5‐di‐( tert ‐butyl)‐1,4‐hydroquinone resulted in a synergistic increase in the magnitude of the mitochondrial Ca 2+ pool. A role for protein ADP‐ribosylation in the intracellular regulation of mitochondrial Ca 2+ homeostasis is suggested.