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Accumulation of the preassembled membrane arm of NADH:ubiquinone oxidoreductase in mitochondria of manganese‐limited grown Neurospora crassa
Author(s) -
Schmidt Michael,
Friedrich Thorsten,
Wallrath Jürgen,
Ohnishi Tomoko,
Weiss Hanns
Publication year - 1992
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(92)81172-i
Subject(s) - neurospora crassa , oxidoreductase , mitochondrion , membrane , manganese , mitochondrial matrix , crassa , biochemistry , neurospora , chemistry , inner mitochondrial membrane , nad+ kinase , biology , biophysics , enzyme , cytosol , gene , organic chemistry , mutant
The NADH:ubiquinone oxidoreductase (complex I) of mitochondria is constructed from two arms arranged perpendicular to each other. The peripheral arm producing into the matrix contains the proximal section of the electron pathway, and the membrane arm with all mitochondrially encoded subunits contains the distal section of the electron pathway. When Neurospora crassa is grown under manganese limitation the formation of the peripheral arm is disturbed, but the membrane arm containing the iron—sulfur cluster N‐2, is accumulated. An extra‐polypeptide, assumed to be a chaperon, is found to be associated with this pre‐assembled membrane arm.